Southeast Asian Journal of Tropical Medicine and Public Health. Vol.12, No.4 (1981), 518-524
Suggested Citation
S. Tharavanij, V. Prasertsiriroj Simultaneous preparation of merozoites and concentrated schizonts of Plasmodium falciparum. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.12, No.4 (1981), 518-524. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30196
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Title
Simultaneous preparation of merozoites and concentrated schizonts of Plasmodium falciparum
A technique is described for a simultaneous preparation of merozoites and concentrated schizonts. The merozoites were harvested after high speed centrifugation of either the supernatant of the low speed centrifugation of the Plasmodium culture or the portion floated in physiogel. The concentrated schizonts were prepared by physiogel floatation technique followed by differential centrifugation in 50 % percoll. An average of 56 % of parasitized red cells were obtained, of which 64 % were schizonts. Concentration of trophozoites and schizonts was 9.5 fold.
