Publication: The F-BAR protein CIP4 inhibits neurite formation by producing lamellipodial protrusions
Issued Date
2012-03-20
Resource Type
ISSN
09609822
Other identifier(s)
2-s2.0-84858698819
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Current Biology. Vol.22, No.6 (2012), 494-501
Suggested Citation
Witchuda Saengsawang, Kelly Mitok, Chris Viesselmann, Lauren Pietila, Derek C. Lumbard, Seth J. Corey, Erik W. Dent The F-BAR protein CIP4 inhibits neurite formation by producing lamellipodial protrusions. Current Biology. Vol.22, No.6 (2012), 494-501. doi:10.1016/j.cub.2012.01.038 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13479
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
The F-BAR protein CIP4 inhibits neurite formation by producing lamellipodial protrusions
Other Contributor(s)
Abstract
Neurite formation is a seminal event in the early development of neurons. However, little is known about the mechanisms by whic h neurons form neurites. F-BAR proteins function in sensing and inducing membrane curvature [1, 2]. Cdc42-interacting protein 4 (CIP4), a member of the F-BAR family, regulates endocytosis in a variety of cell types [3-9] . However, there is little data on how CIP4 functions in neurons [10, 11]. Here we show that CIP4 plays a novel role in neuronal development by inhibiting neurite formation. Remarkably, CIP4 exerts this effect not through endocytosis, but by producing lamellipodial protrusions. In primary cortical neurons CIP4 is concentrated specifically at the tips of extending lamellipodia and filopodia, instead of endosomes as in other cell types. Overexpression of CIP4 results in lamellipodial protrusions around the cell body, subsequently delaying neurite formation and enlarging growth cones. These effects depend on the F-BAR and SH3 domains of CIP4 and on its ability to multimerize. Conversely, cortical neurons from CIP4-null mice initiate neurites twice as fast as controls. This is the first study to demonstrate that an F-BAR protein functions differently in neuronal versus nonneuronal cells and induces lamellipodial protrusions instead of invaginations or filopodia-like structures. © 2012 Elsevier Ltd.