Publication: Synergism of regulatory elements in σ<sup>B</sup>- and σ<sup>A</sup>-dependent promoters enhances recombinant protein expression in Bacillus subtilis
Issued Date
2015-10-01
Resource Type
ISSN
13474421
13891723
13891723
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2-s2.0-84940898015
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Bioscience and Bioengineering. Vol.120, No.4 (2015), 470-475
Suggested Citation
Teva Phanaksri, Plearnpis Luxananil, Sakol Panyim, Witoon Tirasophon Synergism of regulatory elements in σ<sup>B</sup>- and σ<sup>A</sup>-dependent promoters enhances recombinant protein expression in Bacillus subtilis. Journal of Bioscience and Bioengineering. Vol.120, No.4 (2015), 470-475. doi:10.1016/j.jbiosc.2015.02.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35371
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Title
Synergism of regulatory elements in σ<sup>B</sup>- and σ<sup>A</sup>-dependent promoters enhances recombinant protein expression in Bacillus subtilis
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Abstract
© 2015 The Society for Biotechnology, Japan. Strong promoter is an essential factor for production of recombinant protein in various expression systems including Bacillus subtilis. In this study, we described a strategy to improve the expression efficiency using synthetic double promoter. Assembly of the conserved elements from σB- and σA-dependent promoters constitutively improved the yield of recombinant protein approximately 2-3-fold in both exponential and stationary growth phase. The synergistic effect in the double promoter was observed only when σB-promoter was located upstream to σA-promoter but independent to its orientation. A conserved element in either -10 or -35 box of σB-promoter is sufficient to promote the synergism. Hence, this simple strategy of promoter engineering could be an effective way to generate a pool of strong constitutive promoters applicable for heterologous protein expression in B. subtilis in the future.