Publication: Predominant genotypes and alleles of two functional polymorphisms in the manganese superoxide dismutase gene are not associated with Thai cervical or breast cancer
Issued Date
2013
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Language
eng
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Mahidol University
Bibliographic Citation
Asia Pacific Journal of Cancer Prevention. Vol.14, No.6 (2013), 3955-3961
Suggested Citation
Watcharee Attatippaholkun, Kornwipa Wikainapakul Predominant genotypes and alleles of two functional polymorphisms in the manganese superoxide dismutase gene are not associated with Thai cervical or breast cancer. Asia Pacific Journal of Cancer Prevention. Vol.14, No.6 (2013), 3955-3961. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2076
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Title
Predominant genotypes and alleles of two functional polymorphisms in the manganese superoxide dismutase gene are not associated with Thai cervical or breast cancer
Other Contributor(s)
Abstract
Background: Defects of manganese superoxide dismutase (MnSOD) have long been implicated in generation
of oxidative stress and risk susceptibility to various cancers. Two functional polymorphisms within the MnSOD
gene, including the Val-9Ala of the mitochondrial targeting sequence (MTS) and the Ile58Thr of the exon-3,
have been proposed to reduce its enzyme activity and antioxidant potential. Materials and Methods: A highthroughput
multiplex SNaPshot® system was developed herein for simultaneous analyses of Val-9Ala and
Ile58Thr in a single reaction. Genomic DNA extracted from each whole blood sample of 248 patients including
107 with cervical cancer and 141 with breast cancer and from 136 healthy women as controls was analyzed by
the multiplex SNaPshot® system. Results: The Val/Val, Val/Ala genotypes and the Val allele of the MTS were
predominant in patients with cervical or breast cancer as well as healthy women in Thailand. The Ile/Ile genotype
and the Ile allele of the exon-3 were found in all of them whereas none of the Ile/Thr, the Thr/Thr genotypes and
the Thr allele was detected. Genotypic association of both Val-9Ala and Ile58Thr polymorphisms with cervical
cancer and breast cancer of these patients comparing to healthy women was not statistically significant (p<0.05).
Conclusions: The Val/Val, Val/Ala genotypes and the Val allele of the MTS were found predominantly but the
Ile/Ile genotype and the Ile allele of the exon-3 were detected in patients with cervical cancer, breast cancer and
healthy women in Thailand. These two functional polymorphisms (Val-9Ala and Ile58Thr) in MnSOD gene did
not associate with susceptibility risk of these cancer patients in Thailand.
