Glycoalkaloids in potato tubers grown under controlled environments

Abstract

Tuber content of α-solanine, α-chaconine, and total glycoalkaloids (TGA) was determined for the potato cultivars, Norland, Russet Burbank, and Denali grown under different environmental conditions in growth chambers. The lowest TGA concentrations (0.30 to 0.35 mg g-1dry tissue) were found in the cv. Norland with 400 μmol m-2s-1photosynthetic photon flux (PPF), 12 h day length, 16 C temperature, and 350 μmol mol-1carbon dioxide. The ratio of α-chaconine to α-solanine was close to 60:40 under all growing conditions, except that it was 50:50 under the low temperature of 12 C. Cultivars responded similarly to environmental conditions although TGA was about 20% greater in cv. Russet Burbank and about 30% greater in Denali compared to Norland. The largest changes in TGA occurred with changes in temperature. In comparison to 16 C, TGA were 40% greater at 12 C, 80% greater at 20 C, and 125% greater at 24 C (0.70 mg g-1dry weight). The TGA concentratation increased from 10 to 25% with an increase in light from 400 to 800 μmol m-2s-1PPF for all three cultivars. TGA increased 20% with extension of the day length from 12 to 24 hr and also increased 20% when carbon dioxide was increased from 350 to 1000 umol mol-1TGA concentrations were not influenced by changes in relative humidity from 50 to 80%. TGA concentrations decreased only slightly in harvests made from 9 to 21 weeks after planting. Variations in TGA among the different growing conditions and cultivars were below 20 mg/100 g fresh weight (≃ 1.0 mg g-1dry weight) recognized as the upper concentration for food safety. However the results suggest that TGA should be considered when potatoes are grown at temperatures above 20 C.