Publication: Isolation and expression in Escherichia coli of a Xanthomonas oryzae recA-like gene
Issued Date
1993-09-30
Resource Type
ISSN
03781119
Other identifier(s)
2-s2.0-0027439004
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Mahidol University
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SCOPUS
Bibliographic Citation
Gene. Vol.132, No.1 (1993), 113-118
Suggested Citation
Siritida Rabibhadana, Sangpen Chamnongpol, Janine E. Trempy, Nicholas P. Ambulos, Skorn Mongkolsuk Isolation and expression in Escherichia coli of a Xanthomonas oryzae recA-like gene. Gene. Vol.132, No.1 (1993), 113-118. doi:10.1016/0378-1119(93)90522-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22532
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Title
Isolation and expression in Escherichia coli of a Xanthomonas oryzae recA-like gene
Abstract
The recA gene from the bacterium Xanthomonas oryzae pv. oryzae (Xoo), a rice pathogen, was cloned based on its ability to complement DNA repair defects of Escherichia coli recA-mutants. The Xoo recA was localized to a 1.3-kb Sau3AI-XhoI fragment and, when cloned into pBR322, specifies increased methylmethanesulfonate and mitomycin C resistance to E. coli recA mutants and allows λ red-gam-to plaque on an E. coli recA-host. An E. coli recA-strain harboring a plasmid containing the Xoo recA-like gene was shown to produce a 40-kDa protein which cross-reacted with an anti-E. coli RecA antibody. A similar molecular mass protein to RecA has been detected in several Xanthomonas pathovars using an anti-E. coli RecA antibody. Furthermore, the cloned Xoo recA was shown to hybridize to genomic DNA from various Xanthomonas pathovars, but not to genomic DNA from other bacteria species under high-stringency hybridization conditions. These results indicate the isolation of the Xoo recA gene. © 1993.