Publication: An in vitro anti-cancer activity of ocimum tenuiflorum essential oil by inducing apoptosis in human gastric cancer cell line
9
Issued Date
2021-08-01
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ISSN
16489144
1010660X
1010660X
Other identifier(s)
2-s2.0-85112622471
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Mahidol University
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SCOPUS
Bibliographic Citation
Medicina (Lithuania). Vol.57, No.8 (2021)
Suggested Citation
Wongwarut Boonyanugomol, Kamolchanok Rukseree, Pornpan Prapatpong, Onrapak Reamtong, Seung Chul Baik, Myunghwan Jung, Min Kyoung Shin, Hyung Lyun Kang, Woo Kon Lee An in vitro anti-cancer activity of ocimum tenuiflorum essential oil by inducing apoptosis in human gastric cancer cell line. Medicina (Lithuania). Vol.57, No.8 (2021). doi:10.3390/medicina57080784 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/77991
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Title
An in vitro anti-cancer activity of ocimum tenuiflorum essential oil by inducing apoptosis in human gastric cancer cell line
Abstract
The effects of Ocimum tenuiflorum essential oil (OTEO) against gastric cancer remain unknown and merit investigation. In the present study, the anti-cancer activity of OTEO was examined in a human gastric cancer cell line (AGS). After OTEO treatment, AGS cell viability was determined by an MTT assay, and inhibition of metastasis was determined by cell migration and invasion assays. The expression of apoptosis-related genes in treated AGS cells was determined by qRT-PCR. OTEO significantly decreased AGS cell viability in a dose-dependent manner (IC50 163.42 µg/mL) and effectively inhibited cell migration and invasion. Morphological examination demonstrated that OTEO induced cell shrinkage, chromatin condensation, and fragmentation, which are considered typical morphologies of apoptotic cell death. Pro-apoptotic genes (TP53, BAX, and BAK) were significantly up-regulated, while anti-apoptotic genes (BCL-2 and BCL-xL) were significantly down-regulated after treatment with OTEO. In addition, significantly increased gene expression was detected for CASP8, CASP9, and CASP3 in AGS cells exposed to OTEO. GC-MS analysis demonstrated that the major compound of OTEO was caryophyllene (25.85%) and α-pinene (11.66%). This in vitro study demonstrates for the first time that OTEO has potential anti-gastric cancer activity and may induce apoptosis in AGS cells through extrinsic and intrinsic pathways.