Publication: Proteomic profiling of cholangiocarcinoma cell line treated with pomiferin from Derris malaccensis
Issued Date
2005-11-01
Resource Type
ISSN
16159853
Other identifier(s)
2-s2.0-28444463674
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Proteomics. Vol.5, No.17 (2005), 4504-4509
Suggested Citation
Jisnuson Svasti, Chantragan Srisomsap, Pantipa Subhasitanont, Siriporn Keeratichamroen, Daranee Chokchaichamnankit, Lukana Ngiwsara, Nitirat Chimnoi, Somchai Pisutjaroenpong, Supanna Techasakul, Shui Tein Chen Proteomic profiling of cholangiocarcinoma cell line treated with pomiferin from Derris malaccensis. Proteomics. Vol.5, No.17 (2005), 4504-4509. doi:10.1002/pmic.200401315 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16276
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Proteomic profiling of cholangiocarcinoma cell line treated with pomiferin from Derris malaccensis
Other Contributor(s)
Abstract
Pomiferin, a prenylated isoflavonoid from Derris malaccensis with strong anti-fungal and anti-oxidant activities, showed cytotoxic activity towards human cholangiocarcinoma cells (HuCCA-1), with IC50 of 0.9 μg/mL. Pomiferin caused apoptosis, detectable by DNA fragmentation. Two-dimensional PAGE showed increased expression of 12 proteins, namely glucose-regulated protein 75 (grp 75), calcyclin (S100A6), degraded cytokeratin 19, ATP synthase D, ribosomal protein P0, degraded cytokeratin 18 (two spots pI/MW 6.03/29.9 and pI/MW 4.66/21.5), cofilin, annexin A1, triose phosphate isomerase, peroxiredoxin-1, calgizzarin, and profilin. In contrast, cytokeratins (CK) 7, 18 and 19 were down-regulated, and were shown by 1-DE immunodetection to be degraded. © 2005 WILEY-VCH Verlag GmbH & Co. KGaA.