Publication: Effects of Chemical Activation Treatment on Development of Swamp Buffalo (Bubalus bubalis) Oocytes Matured In Vitro and Fertilized by Intracytoplasmic Sperm Injection
Issued Date
2011-02-01
Resource Type
ISSN
14390531
09366768
09366768
Other identifier(s)
2-s2.0-78751494026
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Mahidol University
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SCOPUS
Bibliographic Citation
Reproduction in Domestic Animals. Vol.46, No.1 (2011)
Suggested Citation
Y. Y. Liang, D. N. Ye, C. Laowtammathron, T. Phermthai, T. Nagai, T. Somfai, R. Parnpai Effects of Chemical Activation Treatment on Development of Swamp Buffalo (Bubalus bubalis) Oocytes Matured In Vitro and Fertilized by Intracytoplasmic Sperm Injection. Reproduction in Domestic Animals. Vol.46, No.1 (2011). doi:10.1111/j.1439-0531.2010.01636.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11360
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Title
Effects of Chemical Activation Treatment on Development of Swamp Buffalo (Bubalus bubalis) Oocytes Matured In Vitro and Fertilized by Intracytoplasmic Sperm Injection
Abstract
Contents: The objective of this study was to optimize the activation protocol for buffalo oocytes after intracytoplasmic sperm injection (ICSI). The release of the second polar body (PB) at 3, 6 and 9 h after ICSI of in -vitro matured oocytes activated either with 5 μm ionomycin (Io) or with 7% ethanol (EtOH) was preliminary examined. The highest rate of second PB extrusion occurred at 3 h of activation, and the second PB extrusion in EtOH group was significantly higher than that in Io group. Oocytes that extruded the second PB were selected and cultured either with 1.9 mm 6 -dimethylaminopurine (6 -DMAP) for 3 h or with 10 μg/ml cycloheximide (CHX) for 5 h. Significantly higher rate of oocytes formed 2 pronuclei in EtOH combined with CHX (EtOH + CHX) (62%) group compared to those of Io + CHX (42%) and EtOH + 6 -DMAP (48%) groups (p < 0.01) whereas Io + 6 -DMAP group showed intermediate value (58%). Significantly higher blastocyst formation rates were obtained in Io + 6 -DMAP (29%) and EtOH + CHX (24%) groups than in Io + CHX (6%) and EtOH + 6 -DMAP (17%) groups. Our results indicate that buffalo ICSI oocytes are effectively activated by combination treatment of Io with 6 -DMAP and EtOH with CHX resulting in the highest cleavage and blastocyst formation rates. © 2010 Blackwell Verlag GmbH.