Publication: RAPID ANALYSIS OF CAFFEINE AND PARACETAMOL USING MICRO-LIQUID CHROMATOGRAPHY WITH MIXED MODE MONOLITHIC COLUMN
Issued Date
2021-01-01
Resource Type
ISSN
25870009
0858849X
0858849X
Other identifier(s)
2-s2.0-85126376465
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Mahidol University
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SCOPUS
Bibliographic Citation
Suranaree Journal of Science and Technology. Vol.28, No.2 (2021), 1-6
Suggested Citation
Kesara Ar-sanork, Lalida Wongbuth, Chanpen Karuwan, Nakin Surapanich, Prapin Wilairat, Patcharin Chaisuwan RAPID ANALYSIS OF CAFFEINE AND PARACETAMOL USING MICRO-LIQUID CHROMATOGRAPHY WITH MIXED MODE MONOLITHIC COLUMN. Suranaree Journal of Science and Technology. Vol.28, No.2 (2021), 1-6. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76965
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Title
RAPID ANALYSIS OF CAFFEINE AND PARACETAMOL USING MICRO-LIQUID CHROMATOGRAPHY WITH MIXED MODE MONOLITHIC COLUMN
Abstract
This work developed a simple and rapid micro-liquid chromatographic method using a synthesized monolithic capillary column for analysis of caffeine and paracetamol in pharmaceutical drugs. The monolithic column was synthesized from methacrylic acid and ethylene dimethacrylate monomers. Due to the anionic and hydrophobic properties of the polymeric stationary phase, complete separation of the two compounds was achieved by addition of Methylamine into the acetonitrile-aqueous buffer (pH 10.0) mobile phase using a micro-liquid chromatograph with uv-visible detector. Under the optimal condition, the two compounds were separated in less than 3 min. Good linearity in the concentration range of 1–50 mg/L (r2>0.997) with detection limits of 0.05 mg/L for paracetamol and 0.30 mg/L for caffeine were obtained. The method was successfully applied to the determination of caffeine and paracetamol in drug samples with percent recoveries of 102 and 99, respectively.