Publication: Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents
Issued Date
2008-01-01
Resource Type
ISSN
1574695X
09288244
09288244
Other identifier(s)
2-s2.0-38149111992
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
FEMS Immunology and Medical Microbiology. Vol.52, No.1 (2008), 78-87
Suggested Citation
Chris Druar, Fei Yu, Jodie L. Barnes, Richard T. Okinaka, Narisara Chantratita, Steve Beg, Chad W. Stratilo, Andrew J. Olive, Glenn Soltes, Michelle L. Russell, Direk Limmathurotsakul, Robert E. Norton, Sally X. Ni, William D. Picking, Paul J. Jackson, Don I.H. Stewart, Vadim Tsvetnitsky, Wendy L. Picking, John W. Cherwonogrodzky, Natkunam Ketheesan, Sharon J. Peacock, Erik J. Wiersma Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents. FEMS Immunology and Medical Microbiology. Vol.52, No.1 (2008), 78-87. doi:10.1111/j.1574-695X.2007.00345.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19390
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Title
Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents
Author(s)
Chris Druar
Fei Yu
Jodie L. Barnes
Richard T. Okinaka
Narisara Chantratita
Steve Beg
Chad W. Stratilo
Andrew J. Olive
Glenn Soltes
Michelle L. Russell
Direk Limmathurotsakul
Robert E. Norton
Sally X. Ni
William D. Picking
Paul J. Jackson
Don I.H. Stewart
Vadim Tsvetnitsky
Wendy L. Picking
John W. Cherwonogrodzky
Natkunam Ketheesan
Sharon J. Peacock
Erik J. Wiersma
Fei Yu
Jodie L. Barnes
Richard T. Okinaka
Narisara Chantratita
Steve Beg
Chad W. Stratilo
Andrew J. Olive
Glenn Soltes
Michelle L. Russell
Direk Limmathurotsakul
Robert E. Norton
Sally X. Ni
William D. Picking
Paul J. Jackson
Don I.H. Stewart
Vadim Tsvetnitsky
Wendy L. Picking
John W. Cherwonogrodzky
Natkunam Ketheesan
Sharon J. Peacock
Erik J. Wiersma
Abstract
Burkholderia pseudomallei is a biothreat agent and an important natural pathogen, causing melioidosis in humans and animals. A type III secretion system (TTSS-3) has been shown to be critical for virulence. Because TTSS components from other pathogens have been used successfully as diagnostic agents and as experimental vaccines, it was investigated whether this was the case for BipB, BipC and BipD, components of B. pseudomallei's TTSS-3. The sequences of BipB, BipC and BipD were found to be highly conserved among B. pseudomallei and B. mallei isolates. A collection of monoclonal antibodies (mAbs) specific for each Bip protein was obtained. Most recognized both native and denatured Bip protein. Burkholderia pseudomallei or B. mallei did not express detectable BipB or BipD under the growth conditions used. However, anti-BipD mAbs did recognize the TTSS needle structures of a Shigella strain engineered to express BipD. The authors did not find that BipB, BipC or BipD are protective antigens because vaccination of mice with any single protein did not result in protection against experimental melioidosis. Enzyme-linked immunosorbent assay (ELISA) studies showed that human melioidosis patients had antibodies to BipB and BipD. However, these ELISAs had low diagnostic accuracy in endemic regions, possibly due to previous patient exposure to B. pseudomallei. © 2007 Camgene Corporation.