Publication: Development of Immunochromatographic Test Strip based on Quantum Dot Nanoparticles (QDs)
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Issued Date
2019-10-28
Resource Type
ISSN
1757899X
17578981
17578981
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2-s2.0-85077005503
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Mahidol University
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SCOPUS
Bibliographic Citation
IOP Conference Series: Materials Science and Engineering. Vol.654, No.1 (2019)
Suggested Citation
T. Sioloetwong, A. Kopwitthaya, N. Sathirapongsasuti, K. Nawattanapaiboon, C. Kitiyakara, T. Srikhirin Development of Immunochromatographic Test Strip based on Quantum Dot Nanoparticles (QDs). IOP Conference Series: Materials Science and Engineering. Vol.654, No.1 (2019). doi:10.1088/1757-899X/654/1/012011 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/50828
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Title
Development of Immunochromatographic Test Strip based on Quantum Dot Nanoparticles (QDs)
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Abstract
© Published under licence by IOP Publishing Ltd. Water-soluble fluorescent CdSe/CdZnS quantum dots (QDs) (λem=637nm) were used as a labelling reporter for strip detection. The CdSe/CdZnS QDs was prepared by using core shell technique where CdSe QDs (λem=590nm) core was capped with CdZnS shell. CdZnS-QDs were conjugated with amine-modified DNA probe, that can bio-recognize with DNA product for use in lateral flow assays (LFAs) detection. The presence of functionalized water-dispersible QDs was confirmed by Fourier transform infra-red (FTIR) technique, where a characteristic carboxylic group was observed at 1633 cm-1 (-OH) and 3310 cm-1 (-COO). DNA probe was immobilized onto QDs by EDC/NHS pathways and confirmed by protein absorption spectra at 260 nm due to heterocyclic ring of nucleosides. QDs-DNA can be applied as labelling reporter to readout fluorescence signal on the LFAs detection.