Publication: Influence of fractionation methods on physical and biological properties of injectable platelet-rich fibrin: An exploratory study
Issued Date
2019-04-01
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14220067
16616596
16616596
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2-s2.0-85064922316
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Molecular Sciences. Vol.20, No.7 (2019)
Suggested Citation
Prakan Thanasrisuebwong, Rudee Surarit, Sompop Bencharit, Nisarat Ruangsawasdi Influence of fractionation methods on physical and biological properties of injectable platelet-rich fibrin: An exploratory study. International Journal of Molecular Sciences. Vol.20, No.7 (2019). doi:10.3390/ijms20071657 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50214
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Title
Influence of fractionation methods on physical and biological properties of injectable platelet-rich fibrin: An exploratory study
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Abstract
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Injectable platelet-rich fibrin (i-PRF) has been used as an autografting material to enhance bone regeneration through intrinsic growth factors. However, fractionation protocols used to prepare i-PRF can be varied and the effects of different fractionation protocols are not known. In this study, we investigated the influence of different fractions of i-PRF on the physical and biological properties derived from variations in i-PRF fractionation preparation. The i-PRF samples, obtained from the blood samples of 10 donors, were used to harvest i-PRF and were fractioned into two types. The yellow i-PRF fractionation was harvested from the upper yellow zone, while the red i-PRF fractionation was collected from both the yellow and red zone of the buffy coat. The viscoelastic property measurements, including the clot formation time, α-angle, and maximum clot firmness, were performed by rotational thromboelastometry. The fibrin network was examined using a scanning electron microscope. Furthermore, the concentration of growth factors released, including VEGF, TGF-β1, and PDGF, were quantified using ELISA. A paired t-test with a 95% confidence interval was used. All three viscoelastic properties were statistically significantly higher in the yellow i-PRF compared to the red i-PRF. The scanning electron microscope reviewed more cellular components in the red i-PRF compared to the yellow i-PRF. In addition, the fibrin network of the yellow i-PRF showed a higher density than that in the red i-PRF. There was no statistically significant difference between the concentration of VEGF and TGF-β1. However, at Day 7 and Day 14 PDGF concentrations were statistically significantly higher in the red i-PRF compared to the yellow group. In conclusion, these results showed that the red i-PRF provided better biological properties through the release of growth factors. On the other hand, the yellow i-PRF had greater viscoelastic physical properties. Further investigations into the appropriate i-PRF fractionation for certain surgical procedures are therefore necessary to clarify the suitability for each fraction for different types of regenerative therapy.