Publication:
Luminol encapsulated liposome as a signal generator for the detection of specific antigen-antibody reactions and nucleotide hybridization

Issued Date

2010-07-01

Resource Type

Article

ISSN

13482246
09106340

DOI

10.2116/analsci.26.767

Other identifier(s)

2-s2.0-77955725024

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Analytical Sciences. Vol.26, No.7 (2010), 767-772

Suggested Citation

Pakavadee Rakthong, Akarin Intaramat, Kavi Ratanabanangkoon Luminol encapsulated liposome as a signal generator for the detection of specific antigen-antibody reactions and nucleotide hybridization. Analytical Sciences. Vol.26, No.7 (2010), 767-772. doi:10.2116/analsci.26.767 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28936

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Title

Luminol encapsulated liposome as a signal generator for the detection of specific antigen-antibody reactions and nucleotide hybridization

Author(s)

Pakavadee Rakthong
 Akarin Intaramat
 Kavi Ratanabanangkoon

Other Contributor(s)

Mahidol University
 Chulabhorn Research Institute

Abstract

Liposomes prepared with biotinylated phospholipids and luminol entrapped were shown to be of 187 nm in size, 59% of which were unilamellar and with 43% luminol trapping efficiency. Liposome prepared from biotinylated phospholipids with a longer hydrophilic PEG2000 spacer, but not with the shorter hydrophobic caproyl one, bound efficiently and specifically with immobilized streptavidin in a microplate assay. The interactions of dinitrophenol and tobramycin with their respective antibodies, and the hybridization of 20-mers oligonucleotides were studied using the liposome as a signal generator. These reactions were shown to be specific with limits of detection of 0.58 μM, 0.96 μM and 18 nM, respectively. 2010 © The Japan Society for Analytical Chemistry.

Keyword(s)

Chemistry

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/28936

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Scopus 2006-2010