Publication: Characterization and expression analysis of a Xanthomonas oryzae pv. oryzae recA
Issued Date
1998-01-15
Resource Type
ISSN
03781097
Other identifier(s)
2-s2.0-0032518935
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
FEMS Microbiology Letters. Vol.158, No.2 (1998), 195-200
Suggested Citation
Siritida Rabibhadana, Sangpen Chamnongpol, Rojana Sukchawalit, Nicholas P. Ambulos, Janine E. Trempy, Skorn Mongkolsuk Characterization and expression analysis of a Xanthomonas oryzae pv. oryzae recA. FEMS Microbiology Letters. Vol.158, No.2 (1998), 195-200. doi:10.1016/S0378-1097(97)00522-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18312
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Characterization and expression analysis of a Xanthomonas oryzae pv. oryzae recA
Abstract
Nucleotide sequence of Xanthomonas oryzae pv. oryzae (Xoo) DNA from pSM-A1 was determined and sequence analysis revealed an ORF with high homology to RecA proteins. Expression analysis using an anti-RecA antibody demonstrates that MMS treatment induces recA in trains but not in an Escherichia coli harbouring cloned Xoo recA. This indicates the existence of a recA regulatory mechanism in Xanthomonas that is not function in E. coli. In Xoo, recA was highly induced by treatments with chemical mutagens, UV and peroxides, while superoxides, a thiol agent, a heavy metal and heat shock were not inducers. The increased amount of RecA induced by H2O2or MMS treatments were due to increased transcription of recA. recA showed no growth phase or starvation regulation. The pattern of recA regulation in Xoo could play important roles in stress survival in the environment and during plant-microbe interactions.