In vitro culture of feline embryos increases stress-induced heat shock protein 70 and apoptotic related genes

dc.contributor.authorThanida Sananmuangen_US
dc.contributor.authorNawapen Phutikaniten_US
dc.contributor.authorCatherine Nguyenen_US
dc.contributor.authorSukanya Manee-Inen_US
dc.contributor.authorMongkol Techakumphuen_US
dc.contributor.authorTheerawat Tharasaniten_US
dc.contributor.otherChulalongkorn Universityen_US
dc.contributor.otherTechnologie Avancee pour le Genome et la Cliniqueen_US
dc.contributor.otherMahidol Universityen_US
dc.description.abstractDevelopmental competence and quality of in vitro produced embryos has been demonstrated to be lower than in vivo derived embryos. This study aimed specifically to determine the effects of in vitro culture of feline embryos using various culture densities on developmental competence and expression of stress- and apoptotic-related genes in terms of heat shock protein 70 (HSP70) and apoptotic-related (BAX and BCL-2) gene expressions. In experiment 1, we characterized the inducible form of a feline-specific HSP70 mRNA sequence, as it has not been previously reported. The primers for feline HSP70 mRNA were synthesized and tested on heat-treated cat fibroblasts. In experiment 2, feline embryos were cultured at different culture densities (embryo: culture volume; 1:1.25, 1:5 and 1:20). The developmental competence was determined along with HSP70, BAX and BCL-2 transcript abundances using quantitative RT-PCR. In vivo derived embryos were used as a control group. A partial cat HSP70 mRNA sequence (190 bp) was characterized and exhibited high nucleotide identity (93 to 96%) with other species. Cleaved embryos cultured at high density (1:1.25) developed to blastocysts at a lower rate than those generated from lower densities. Irrespective of the culture densities used, in vitro cultured blastocysts showed increased levels of HSP70 and BAX transcripts compared with in vivo counterparts. Blastocysts derived from the highest culture density (1:1.25) showed higher levels of upregulation of HSP70 and BAX transcripts than those cultured at lower culture densities (1:5 and 1:20). In conclusion, increased levels of pro-apoptotic (BAX) and stress-response (HSP70) transcripts correlated with developmental incompetence of embryos cultured at high embryonic density, indicating that stress accumulated during in vitro embryo culture affected the fate for embryo development and quality. © 2013 by the Society for Reproduction and Development.en_US
dc.identifier.citationJournal of Reproduction and Development. Vol.59, No.2 (2013), 180-188en_US
dc.rightsMahidol Universityen_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.titleIn vitro culture of feline embryos increases stress-induced heat shock protein 70 and apoptotic related genesen_US