Publication: Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries
Issued Date
2013-12-01
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ISSN
14602091
03057453
03057453
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2-s2.0-84887517155
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Antimicrobial Chemotherapy. Vol.68, No.12 (2013), 2820-2824
Suggested Citation
Moon Jung Kim, Il Kwon Bae, Seok Hoon Jeong, So Hyun Kim, Jae Hoon Song, Jae Young Choi, Sang Sun Yoon, Visanu Thamlikitkul, Po Ren Hsueh, Rohani Md Yasin, M. K. Lalitha, Kyungwon Lee Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries. Journal of Antimicrobial Chemotherapy. Vol.68, No.12 (2013), 2820-2824. doi:10.1093/jac/dkt269 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/32070
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Title
Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries
Abstract
Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the blaVIM-2(n=12), blaVIM-3(n=1), blaIMP-6(n=2) and blaIMP-26(n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries. © The Author 2013. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.