Publication: Chiral separation of pheniramine by capillary electrophoresis partial-filling technique using hydroxypropyl-β-cyclodextrin as chiral selector
Issued Date
2010-08-01
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ISSN
10619348
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2-s2.0-78651443081
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Analytical Chemistry. Vol.65, No.8 (2010), 803-808
Suggested Citation
K. Phatthiyaphaibun, W. Som-Aum, M. Srisa-Ard, J. Threeprom Chiral separation of pheniramine by capillary electrophoresis partial-filling technique using hydroxypropyl-β-cyclodextrin as chiral selector. Journal of Analytical Chemistry. Vol.65, No.8 (2010), 803-808. doi:10.1134/S106193481008006X Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28930
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Title
Chiral separation of pheniramine by capillary electrophoresis partial-filling technique using hydroxypropyl-β-cyclodextrin as chiral selector
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Abstract
A simple capillary electrophoresis partial-filling technique for the enantioseparation of pheniramine is presented. Phosphate buffer and hydroxypropyl-β-cyclodextrin (HP-β-CD) were used as the electrolyte and chiral selector, respectively. Several experimental parameters such as HP-β-CD concentration, HP-β-CD plug length, CE temperature and applied voltage were studied. Under the selected conditions, pheniramine enantiomers can be separated within less than 14 min. The assay was validated for linearity (5.0 × 10-6-5.0 × 10-4M; R2= 0.9987), limit of detection (5.0 × 10-7M), limit of quantitation (5.0 × 10-6M), analytical precision (%RSD ≤ 9.8) and accuracy (%recovery = 101 ± 3). The proposed methodology was then applied to the analysis of a commercially available pharmaceutical eye drop preparation. The results are in good agreement with that declared by the manufacturer. The proposed methodology provides adequate results in terms of simplicity, cost, sample throughput, repeatability and accuracy for quality control of pheniramine enantiomers in pharmaceutical preparations. © Pleiades Publishing, Ltd., 2010.