Publication: Prevalence and Multidrug Resistance of Salmonella in Swine Production Chain in a Central Province, Thailand
Issued Date
2021-12-01
Resource Type
ISSN
19449097
0362028X
0362028X
Other identifier(s)
2-s2.0-85122048280
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Food Protection. Vol.84, No.12 (2021), 2174-2184
Suggested Citation
Kaknokrat Chonsin, Ruchirada Changkwanyeun, Achiraya Siriphap, Apiradee Intarapuk, Watsawan Prapasawat, Kanjana Changkaew, Chaiwat Pulsrikarn, Norikazu Isoda, Chie Nakajima, Yasuhiko Suzuki, Orasa Suthienkul Prevalence and Multidrug Resistance of Salmonella in Swine Production Chain in a Central Province, Thailand. Journal of Food Protection. Vol.84, No.12 (2021), 2174-2184. doi:10.4315/JFP-21-003 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/75513
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Title
Prevalence and Multidrug Resistance of Salmonella in Swine Production Chain in a Central Province, Thailand
Abstract
Salmonella causes foodborne disease outbreaks worldwide and raises concerns about public health and economic losses. To determine prevalence, serovar, antimicrobial resistance patterns, and the presence of extended-spectrum β-lactamase (ESBL) genes in a cross-sectional study, 418 total samples from feces and carcasses (from three slaughterhouses) and pork and cutting boards (from four markets) were collected in a central Thailand province in 2017 and 2018. Of the 418 samples, 272 (65.1%) were positive for Salmonella. The prevalence of Salmonella-positive samples from markets (158 of 178; 88.8%) was significantly higher than that among samples from slaughterhouses (114 of 240; 47.5%) (P < 0.05). A total of 1,030 isolates were identified; 409 were assigned to 45 serovars, with Salmonella Rissen the most common (82 of 409; 20%). Two serovars, Salmonella Cannstatt and Salmonella Braubach, were identified for the first time in Thailand in market and slaughterhouse samples, respectively. Among 180 isolates representing 19 serovars, 133 (73.9%) exhibited multidrug resistance. Screening for ESBL production revealed that 41 (10.3%) of 399 isolates were ESBL positive. The prevalence of ESBL-producing Salmonella isolates was significantly higher among the market isolates (31 of 41; 75.6%) than among the slaughterhouse isolates in (10 of 41; 24.4%) (P < 0.05). In market samples, 24 (77.4%) of 31 isolates were recovered from pork and 7 (22.6%) were recovered from cutting boards. Nine ESBL-producing isolates carried single ESBL genes, either blaTEM (4 of 41 isolates; 9.8%) or blaCTX-M (5 of 41 isolates; 12.2%), whereas 11 (26.8%) carried both blaTEM and blaCTX-M. No ESBL-producing Salmonella isolate carried the blaSHV gene. These results suggest that pigs, their flesh, and cutting boards used for processing pork could be reservoirs for widespread ESBL-producing Salmonella isolates with multidrug resistance and outbreak potential across the food chain.