Publication: Transmission of Francisella noatuensis subsp. orientalis from subclinically infected hybrid red tilapia broodstock (Oreochromis sp.) to their offspring
Issued Date
2019-11-01
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ISSN
10961208
08824010
08824010
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2-s2.0-85071400957
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Mahidol University
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SCOPUS
Bibliographic Citation
Microbial Pathogenesis. Vol.136, (2019)
Suggested Citation
Vuong Viet Nguyen, Ha Thanh Dong, Saengchan Senapin, Warachin Gangnonngiw, Nopadon Pirarat, Pattanapon Kayansamruaj, Tilladit Rung-ruangkijkrai, Channarong Rodkhum Transmission of Francisella noatuensis subsp. orientalis from subclinically infected hybrid red tilapia broodstock (Oreochromis sp.) to their offspring. Microbial Pathogenesis. Vol.136, (2019). doi:10.1016/j.micpath.2019.103670 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50999
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Title
Transmission of Francisella noatuensis subsp. orientalis from subclinically infected hybrid red tilapia broodstock (Oreochromis sp.) to their offspring
Abstract
© 2019 Elsevier Ltd Francisella noatunensis subsp. orientalis (Fno) has been reported as an important bacterial pathogen causing significant mortality (30–95%) in farmed tilapia in broad geographic areas. However, we found that there was a proportion of broodfish in our laboratory that appeared to be healthy but which tested positive for Fno. We therefore hypothesized that Fno might be able to be transmitted from subclinically infected tilapia mouthbrooders to their offspring through the current practice of fry production in tilapia hatcheries. To prove this, experimentally infected hybrid red tilapia broodstock were mated and their offspring were examined for the presence of Fno. In this study, three pairs of infected broodfish were mated for natural spawning and fertilized eggs from each couple were then collected from the female mouths for artificial incubation. The newly hatched larvae were cultured for 30 days and sample collection was performed at different developmental stages i.e. yolk-sac larvae, 5 and 30-day old fry. The results showed that the ovary and testis of all 3 pairs of the broodstock, as well as their fertilized eggs and offspring were Fno positive by Fno-specific PCR and in situ DNA hybridization. In summary, this study revealed that with the current practice in tilapia hatcheries, Fno might be able to transmit from subclinically infected tilapia mouthbrooders to their offspring. Therefore, using Fno-free broodfish in tilapia hatcheries should be considered in order to produce Fno-free tilapia fry.