Publication: Rapid idenfication of Burkholderia pseudomallei in blood culture supernatants by a coagglutination assay
Issued Date
2005-01-01
Resource Type
ISSN
14690691
1198743X
1198743X
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2-s2.0-27544472952
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinical Microbiology and Infection. Vol.11, No.11 (2005), 930-933
Suggested Citation
M. V. Jesudason, V. Balaji, S. Sirisinha, G. Sridharan Rapid idenfication of Burkholderia pseudomallei in blood culture supernatants by a coagglutination assay. Clinical Microbiology and Infection. Vol.11, No.11 (2005), 930-933. doi:10.1111/j.1469-0691.2005.01235.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17093
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Title
Rapid idenfication of Burkholderia pseudomallei in blood culture supernatants by a coagglutination assay
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Abstract
In total, 309 blood culture supernatants were tested for the presence of Burkholderia pseudomallei antigen using an in-house coagglutination test prepared by sensitising Cowan I staphylococcal. cells with B. pseudomallei polyclonal antiserum. The coagglutination test gave a sensitivity, specificity, positive predictive value and negative predictive value of 100% in comparison with blood culture. A subset of 102 supernatants was also tested for B. pseudomallei antigen using a monoclonal antibody-based latex agglutination test. The sensitivity, specificity, positive predictive value and negative predictive values of this test were 100%, 90%, 75% and 100%, respectively. © 2005 Copyright by the European Society of Clinical Microbiology and Infectious Diseases.