Publication: Detection of salivary antibodies to crude antigens of Opisthorchis viverrini in opisthorchiasis and cholangiocarcinoma patients
Issued Date
2011-08-01
Resource Type
ISSN
14363771
14326981
14326981
Other identifier(s)
2-s2.0-79960134115
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Clinical Oral Investigations. Vol.15, No.4 (2011), 477-483
Suggested Citation
Ponlatham Chaiyarit, Paiboon Sithithaworn, Chanitra Thuwajit, Puangrat Yongvanit Detection of salivary antibodies to crude antigens of Opisthorchis viverrini in opisthorchiasis and cholangiocarcinoma patients. Clinical Oral Investigations. Vol.15, No.4 (2011), 477-483. doi:10.1007/s00784-010-0421-y Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11824
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Detection of salivary antibodies to crude antigens of Opisthorchis viverrini in opisthorchiasis and cholangiocarcinoma patients
Other Contributor(s)
Abstract
Opisthorchis viverrini (O. viverrini; known as human liver fluke) is a major health problem in the northeastern region of Thailand. Infection with O. viverrini is the cause of hepatobiliary disease and cholangiocarcinoma (CCA). Previous studies demonstrated specific antibodies to crude O. viverrini antigens in serum from O. viverrini-infected patients. However, no studies have measured specific antibodies to O. viverrini antigens in saliva from patients with opisthorchiasis and CCA. The objective of the study was to detect specific antibodies to crude O. viverrini antigens in saliva from patients with opisthorchiasis and CCA, and to evaluate their use for diagnosis of O. viverrini infection. Saliva samples from 23 control subjects, 30 opisthorchiasis patients, and 38 CCA patients were collected. ELISA was established for detection of salivary IgA and IgG to crude O. viverrini antigens. ANOVA was used to compare salivary IgA and IgG levels among groups. Salivary IgA to crude O. viverrini antigens in CCA patients was significantly higher than controls (p = 0.007). Salivary IgG in CCA patients was significantly higher than opisthorchiasis patients and controls (p = 0.010 and p < 0.001, respectively). The cut-off value from salivary IgG test demonstrated higher accuracy for positivity of O. viverrini infection than salivary IgA. In conclusion, specific antibodies to crude O. viverrini antigens were detected in saliva of patients with opisthorchiasis and CCA. Salivary antibodies reflect serum immune response to O. viverrini infection, and salivary IgG tends to be a good candidate for diagnosis of O. viverrini infection. © 2010 Springer-Verlag.