Publication: Molecular characterization identifies intra-host recombination and zoonotic potential of canine rotavirus among dogs from Thailand
Issued Date
2020-01-01
Resource Type
ISSN
18651682
18651674
18651674
Other identifier(s)
2-s2.0-85089963485
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Transboundary and Emerging Diseases. (2020)
Suggested Citation
Kamonpan Charoenkul, Taveesak Janetanakit, Napawan Bunpapong, Supanat Boonyapisitsopa, Ratanaporn Tangwangvivat, Kamol Suwannakarn, Apiradee Theamboonlers, Yong Poovorawan, Alongkorn Amonsin Molecular characterization identifies intra-host recombination and zoonotic potential of canine rotavirus among dogs from Thailand. Transboundary and Emerging Diseases. (2020). doi:10.1111/tbed.13778 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/59130
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Molecular characterization identifies intra-host recombination and zoonotic potential of canine rotavirus among dogs from Thailand
Other Contributor(s)
Abstract
© 2020 Wiley-VCH GmbH From September 2016 to January 2019, we collected 710 rectal swabs from both healthy and sick dogs from small animal hospitals in 5 provinces of Thailand. The samples were tested for canine rotavirus group A (CRV) by using one-step RT-PCR specific to the VP6 gene. Our results showed that 0.70% (5/710) were positive for CRV. The five CRVs were then characterized by whole-genome sequencing. Our results showed that the genotype of Thai CRVs is G3P[3], which is the predominant genotype reported in dogs. The Thai CRVs posed a novel genetic constellation ‘G3-P[3]-I3-R3-C3-M3-A9-N2-T3-E3-H6’, which has never been reported in CRVs from dogs but has been reported in rotaviruses from humans. Based on phylogenetic analysis, the Thai CRVs are the result of multiple reassortments in which gene segments might have originated from human and bat rotaviruses and suggests the zoonotic potential of the virus.