Publication: Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos
Issued Date
2008-01-01
Resource Type
ISSN
07328893
Other identifier(s)
2-s2.0-37049008638
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Mahidol University
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SCOPUS
Bibliographic Citation
Diagnostic Microbiology and Infectious Disease. Vol.60, No.1 (2008), 43-49
Suggested Citation
Stuart D. Blacksell, Mammen P. Mammen, Soulignasack Thongpaseuth, Robert V. Gibbons, Richard G. Jarman, Kemajittra Jenjaroen, Ananda Nisalak, Rattanaphone Phetsouvanh, Paul N. Newton, Nicholas P.J. Day Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. Diagnostic Microbiology and Infectious Disease. Vol.60, No.1 (2008), 43-49. doi:10.1016/j.diagmicrobio.2007.07.011 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19829
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Title
Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos
Abstract
We evaluated 2 commercial enzyme-linked immunosorbent assays (ELISAs) for the diagnosis of dengue infection; one a serologic test for immunoglobulin M (IgM) antibodies, the other based on detection of dengue virus nonstructural 1 (NS1) antigen. Using gold standard reference serology on paired sera, 41% (38/92 patients) were dengue confirmed, with 4 (11%) acute primary and 33 (87%) acute secondary infections (1 was of indeterminate status). Sensitivity of the NS1-ELISA was 63% (95% confidence interval [CI], 53-73) on admission samples but was much less sensitive (5%; 95% CI, 1-10) on convalescent samples. The IgM capture ELISA had a lower but statistically equivalent sensitivity compared with the NS1-ELISA for admission samples (45%; 95% CI, 35-55) but was more sensitive on convalescent samples (58%; 95% CI, 48-68). The results of the NS1 and IgM capture ELISAs were combined using a logical OR operator, increasing the sensitivity for admission samples (79%; 95% CI, 71-87), convalescent samples (63%; 95% CI, 53-73), and all samples (71%; 95% CI, 65-78). © 2008 Elsevier Inc. All rights reserved.