Publication: Allergen components of Dermatophagoides farinae recognised by serum immunoglobulin (Ig)E in Thai dogs with atopic dermatitis
Issued Date
2021-08-01
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ISSN
13653164
09594493
09594493
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2-s2.0-85105932736
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Mahidol University
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SCOPUS
Bibliographic Citation
Veterinary Dermatology. Vol.32, No.4 (2021), 338-e94
Suggested Citation
Nathrada Khantavee, Onrapak Reamtong, Nitat Sookrung, Sanipa Suradhat, Nuvee Prapasarakul Allergen components of Dermatophagoides farinae recognised by serum immunoglobulin (Ig)E in Thai dogs with atopic dermatitis. Veterinary Dermatology. Vol.32, No.4 (2021), 338-e94. doi:10.1111/vde.12967 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/79153
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Title
Allergen components of Dermatophagoides farinae recognised by serum immunoglobulin (Ig)E in Thai dogs with atopic dermatitis
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Abstract
Background: Dermatophagoides farinae (Der f) is a common allergen in dogs with atopic dermatitis (AD). However, the relevant components of Der f require further investigation. Objectives: We aimed to provide data on the immunoglobulin (Ig)E-binding specific components of Der f for further diagnostic and therapeutic applications. Animals: Serum samples were collected from five healthy, nine Der f-allergic atopic and seven non-Der f-allergic atopic dogs identified based on an intradermal skin test. Methods and materials: We explored the component profiles of Der f extracts through 2D gel electrophoresis and IgE immunoblotting. The IgE-binding components in both groups of atopic dogs were analysed by mass spectrometry. Results: The majority of Der f-allergic atopic dogs recognised Der f Alternaria alternata allergen 10 (Der f Alt a 10), elongation factor 1-alpha (EF1-α), gelsolin-like allergen Der f 16, Der f 28 and Der f 2. Der f 3, Der f 10, Der f 20 and Der f 32 were recognised as minor allergens. Alpha-enolase, serine protease, arginine kinase and a few hypothetical proteins were recognised components in both groups of atopic dogs. Unexpectedly, Der f 15 (chitinase) was found to be a minor component. Conclusions and clinical importance: Multiple IgE-binding allergens of Der f were identified in Thai atopic dogs. We propose that the specific antigen set that is bound by IgE, comprising Der f Alt a 10, EF1-α, gelsolin-like Der f 16, Der f 28 and Der f 2, could be used for future diagnostics and immunotherapy platforms.