Publication: Identification and antimicrobial susceptibility of microorganisms isolated from severe corneal ulcers of dogs in Thailand
Issued Date
2018-08-01
Resource Type
ISSN
13477439
09167250
09167250
Other identifier(s)
2-s2.0-85052586206
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Veterinary Medical Science. Vol.80, No.8 (2018), 1259-1265
Suggested Citation
Daneeya Ekapopphan, Athicha Srisutthakarn, Walasinee Moonarmart, Ruangrat Buddhirongawatr, Norasuthi Bangphoomi Identification and antimicrobial susceptibility of microorganisms isolated from severe corneal ulcers of dogs in Thailand. Journal of Veterinary Medical Science. Vol.80, No.8 (2018), 1259-1265. doi:10.1292/jvms.18-0045 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/47456
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Identification and antimicrobial susceptibility of microorganisms isolated from severe corneal ulcers of dogs in Thailand
Other Contributor(s)
Abstract
©2018 The Japanese Society of Veterinary Science. This study aims to determine the microbiological profile and risk factors associated with antimicrobial-resistant bacteria in canine severe corneal ulcers. Thirty-two corneal and conjunctival swabs were collected from dogs with diagnosed severe corneal ulcers that presented to Prasu-Arthorn veterinary teaching hospital in Nakhon Pathom, Thailand from June 2015 to June 2016. Microorganisms were identified by means of genotypic and phenotypic approaches. Of 32 ulcers sampled, 26 (81.3%) yielded culturable microorganisms with 24 bacterial isolates and 7 fungal isolates. The most commonly isolated bacteria were Staphylococcus spp. (45.8%, 11/24) and Pseudomonas aeruginosa (20.8%, 5/24). Out of 11 staphylococcal isolates identified, 10 carried the mecA gene providing methicillin resistance. The extended-spectrum β-lactamase (ESBL) encoding genes bla CTX-M and bla VEB-1 were found in an Acinetobacter lwoffii isolate, and bla SHV was found in a P. aeruginosa isolate. Based on the Clinical Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoint criteria, minimum inhibitory concentrations values showed that all bacteria, except for staphylococci, were susceptible to current ophthalmic antibiotics. More than 50% of staphylococci were resistant to all generations of fluoroquinolones and fusidic acid. Chloramphenicol was highly active against staphylococci (81.3% susceptible). The width (P=0.02) and the depth (P=0.04) of ulcers predicted greater risk of yielding resistant bacteria. The identification of antimicrobial-resistant bacteria prompts practitioners to be prudent when choosing ophthalmic antibiotics for severe corneal ulcers.