Journal of Applied Microbiology. Vol.89, No.1 (2000), 152-157
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W. Panbangred, K. Weeradechapon, S. Udomvaraphant, K. Fujiyama, V. Meevootisom High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant. Journal of Applied Microbiology. Vol.89, No.1 (2000), 152-157. doi:10.1046/j.1365-2672.2000.01093.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/25860
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High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant
By marker exchange mutagenesis, Bacillus megaterium strain UN-1 (Bm-UN1) was used to prepare a mutant strain B. megaterium UN-cat (Bm-UNcat) lacking the penicillin G acylase gene (pac). The pac gene from Bm-UN1 was subcloned into pTF6 and the resultant plasmid, pBA402, was introduced into Bm-UNcat and Bacillus subtilis. Bm-UNcat harbouring pBA402 produced high penicillin G acylase (PAC) activity of 13.7, 19.5 and 20.4 U ml1 at 24, 36 and 48 h of culture, respectively. This was two- to fivefold higher than PAC produced by B. subtilis harbouring pBA402 and about 20-fold higher than PAC produced by the parent strain, Bm-UN1.
