Publication: DNA Sequence Preferences of Transcriptional Activators Correlate More Strongly than Repressors with Nucleosomes
Issued Date
2012-07-27
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ISSN
10974164
10972765
10972765
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2-s2.0-84864300296
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular Cell. Vol.47, No.2 (2012), 183-192
Suggested Citation
Varodom Charoensawan, Sarath Chandra Janga, Martha L. Bulyk, M. Madan Babu, Sarah A. Teichmann DNA Sequence Preferences of Transcriptional Activators Correlate More Strongly than Repressors with Nucleosomes. Molecular Cell. Vol.47, No.2 (2012), 183-192. doi:10.1016/j.molcel.2012.06.028 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13660
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Title
DNA Sequence Preferences of Transcriptional Activators Correlate More Strongly than Repressors with Nucleosomes
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Abstract
Transcription factors (TFs) and histone octamers are two abundant classes of DNA binding proteins that coordinate the transcriptional program in cells. Detailed studies of individual TFs have shown that TFs bind to nucleosome-occluded DNA sequences and induce nucleosome disruption/repositioning, while recent global studies suggest this is not the only mechanism used by all TFs. We have analyzed to what extent the intrinsic DNA binding preferences of TFs and histones play a role in determining nucleosome occupancy, in addition to nonintrinsic factors such as the enzymatic activity of chromatin remodelers. The majority of TFs in budding yeast have an intrinsic sequence preference overlapping with nucleosomal histones. TFs with intrinsic DNA binding properties highly correlated with those of histones tend to be associated with gene activation and might compete with histones to bind to genomic DNA. Consistent with this, we show that activators induce more nucleosome disruption upon transcriptional activation than repressors. © 2012 Elsevier Inc.