Publication: The impact of culture dimensionality on behavioral epigenetic memory contributing to pluripotent state of iPS cells
Issued Date
2020-01-01
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ISSN
10974652
00219541
00219541
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2-s2.0-85097484834
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Cellular Physiology. (2020)
Suggested Citation
Naruchit Thanuthanakhun, Masahiro Kino-oka, Suparerk Borwornpinyo, Yuzuru Ito, Mee Hae Kim The impact of culture dimensionality on behavioral epigenetic memory contributing to pluripotent state of iPS cells. Journal of Cellular Physiology. (2020). doi:10.1002/jcp.30211 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/60406
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Title
The impact of culture dimensionality on behavioral epigenetic memory contributing to pluripotent state of iPS cells
Abstract
© 2020 Wiley Periodicals LLC Three-dimensional (3D) culture platforms have been explored to establish physiologically relevant cell culture environment and permit expansion scalability; however, little is known about the mechanisms underlying the regulation of pluripotency of human induced pluripotent stem cells (hiPSCs). This study elucidated epigenetic modifications contributing to pluripotency of hiPSCs in response to 3D culture. Unlike two-dimensional (2D) monolayer cultures, 3D cultured cells aggregated with each other to form ball-like aggregates. 2D cultured cells expressed elevated levels of Rac1 and RhoA; however, Rac1 level was significantly lower while RhoA level was persisted in 3D aggregates. Compared with 2D monolayers, the 3D aggregates also exhibited significantly lower myosin phosphorylation. Histone methylation analysis revealed remarkable H3K4me3 upregulation and H3K27me3 maintenance throughout the duration of 3D culture; in addition, we observed the existence of naïve pluripotency signatures in cells grown in 3D culture. These results demonstrated that hiPSCs adapted to 3D culture through alteration of the Rho-Rho kinase-phospho-myosin pathway, influencing the epigenetic modifications and transcriptional expression of pluripotency-associated factors. These results may help design culture environments for stable and high-quality hiPSCs.