Publication: Effect of temperature on fimbrial gene expression and adherence of enteroaggregative Escherichia coli
Issued Date
2015-07-23
Resource Type
ISSN
16604601
16617827
16617827
Other identifier(s)
2-s2.0-84937835174
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Environmental Research and Public Health. Vol.12, No.8 (2015), 8631-8643
Suggested Citation
Woranich Hinthong, Nitaya Indrawattana, Pannamthip Pitaksajjakul, Chonlatip Pipattanaboon, Thida Kongngoen, Prapin Tharnpoophasiam, Suwalee Worakhunpiset Effect of temperature on fimbrial gene expression and adherence of enteroaggregative Escherichia coli. International Journal of Environmental Research and Public Health. Vol.12, No.8 (2015), 8631-8643. doi:10.3390/ijerph120808631 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35990
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Effect of temperature on fimbrial gene expression and adherence of enteroaggregative Escherichia coli
Other Contributor(s)
Abstract
© 2015 by the authors; licensee MDPI, Basel, Switzerland. The influence of temperature on bacterial virulence has been studied worldwide from the viewpoint of climate change and global warming. The bacterium enteroaggregative Escherichia coli (EAEC) is the causative agent of watery diarrhea and shows an increasing incidence worldwide. Its pathogenicity is associated with the virulence factors aggregative adherence fimbria type I and II (AAFI and AAFII), encoded by aggA and aafA in EAEC strains 17-2 and 042, respectively. This study focused on the effect of temperature increases from 29 °C to 40 °C on fimbrial gene expression using real-time PCR, and on its virulence using an aggregative adherence assay and biofilm formation assay. Incubation at 32 °C caused an up-regulation in both EAEC strains 17-2 and strain 042 virulence gene expression. EAEC strain 042 cultured at temperature above 32 °C showed down-regulation of aafA expression except at 38 °C. Interestingly, EAEC cultured at a high temperature showed a reduced adherence to cells and an uneven biofilm formation. These results provide evidence that increases in temperature potentially affect the virulence of pathogenic EAEC, although the response varies in each strain.