Publication: Attenuating characteristics of DEN-2 PDK53 in flavivirus-naïve peripheral blood mononuclear cells
Issued Date
2007-05-10
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ISSN
0264410X
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2-s2.0-34247203627
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Mahidol University
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SCOPUS
Bibliographic Citation
Vaccine. Vol.25, No.19 (2007), 3896-3905
Suggested Citation
Jundee Rabablert, Chantapong Wasi, Richard Kinney, Jitra Kasisith, Dhanesh Pitidhammabhorn, Sukathida Ubol Attenuating characteristics of DEN-2 PDK53 in flavivirus-naïve peripheral blood mononuclear cells. Vaccine. Vol.25, No.19 (2007), 3896-3905. doi:10.1016/j.vaccine.2007.01.096 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24553
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Title
Attenuating characteristics of DEN-2 PDK53 in flavivirus-naïve peripheral blood mononuclear cells
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Abstract
A live-attenuated DEN-2 virus, DEN-2 strain 16681-PDK53, has been found to be attenuated for both humans and mice with an unknown mechanism. To partially answer this question, responses of flavivirus-naïve primary human PBMC to infection with attenuated DEN-2 PDK53 (D2/IC-VV45R) virus and its parental, virulent DEN-2 16681 virus (D2/IC-30P-A) were investigated at the cellular and genetic levels using cDNA array analysis. Both DEN-2 viruses produced similar replication kinetics in flavivirus-naïve PBMC. In contrast, virulent DEN-2 virus caused a higher percentage of apoptotic death. A macro-array analysis showed that the virulent D2/IC-30P-A virus induced changes in the expression of a greater number of genes than did the attenuated D2/IC-VV45R virus, 31 genes versus 19 genes, respectively, by 24 h post-infection. Interestingly, both viruses stimulated cytokines known to be virulence factors for DEN virus infection, such as IL-1β, IL-6, IL-8, IL-10, MIP-1β, and MIP-1α. The virulent virus additionally up-regulates immune suppression factors and down-regulates immune activator and growth factors. In conclusion, our data demonstrated that D2-PDK53 effected less change in PBMC than D2-16681 in terms of observable cellular effect and expression of cytokine and chemokine related genes. © 2007 Elsevier Ltd. All rights reserved.