Publication:
A plasmid encoding a combination of mosquito-larvicidal genes from Bacillus thuringiensis subsp. israelensis and Bacillus sphaericus confers toxicity against a broad range of mosquito larvae when expressed in Gram-negative bacteria

Issued Date

2003-11-21

Resource Type

Article

ISSN

03781097

DOI

10.1016/S0378-1097(03)00780-8

Other identifier(s)

2-s2.0-0344153762

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

FEMS Microbiology Letters. Vol.228, No.2 (2003), 259-263

Suggested Citation

Sutipa Tanapongpipat, Plearnpis Luxananil, Boonhiang Promdonkoy, Namchai Chewawiwat, Mongkon Audtho, Sakol Panyim A plasmid encoding a combination of mosquito-larvicidal genes from Bacillus thuringiensis subsp. israelensis and Bacillus sphaericus confers toxicity against a broad range of mosquito larvae when expressed in Gram-negative bacteria. FEMS Microbiology Letters. Vol.228, No.2 (2003), 259-263. doi:10.1016/S0378-1097(03)00780-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20678

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Title

A plasmid encoding a combination of mosquito-larvicidal genes from Bacillus thuringiensis subsp. israelensis and Bacillus sphaericus confers toxicity against a broad range of mosquito larvae when expressed in Gram-negative bacteria

Author(s)

Sutipa Tanapongpipat
 Plearnpis Luxananil
 Boonhiang Promdonkoy
 Namchai Chewawiwat
 Mongkon Audtho
 Sakol Panyim

Other Contributor(s)

Thailand National Center for Genetic Engineering and Biotechnology
 Mahidol University

Abstract

A recombinant plasmid harboring cry4A, cry4B and cry11A from Bacillus thuringiensis subsp. israelensis and binary toxin genes from Bacillus sphaericus has been constructed. The three cry genes were placed under the control of the cry4B promoter whereas the binary toxin gene was controlled by its native promoter. The expression of toxins in Escherichia coli harboring the resulting plasmid, p4BDA-5142, was investigated. Cry4B expression was highest compared to other toxins. Although the level of toxin expression was low compared with E. coli expressing single toxins, the recombinant E. coli strain harboring p4BDA-5142 exhibited broad range mosquito-larvicidal activity against all Aedes, Culex and Anopheles larvae. This work has shown that the development of the recombinant plasmid can be used to broaden the host range spectrum of the appropriate bacterial host for mosquito control. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Keyword(s)

Biochemistry, Genetics and Molecular Biology
 Immunology and Microbiology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/20678

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