Publication: Enhanced chondrogenic differentiation of dental pulp stem cells using nanopatterned PEG-GelMA-HA hydrogels
Issued Date
2014-01-01
Resource Type
ISSN
1937335X
19373341
19373341
Other identifier(s)
2-s2.0-84909959291
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Mahidol University
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SCOPUS
Bibliographic Citation
Tissue Engineering - Part A. Vol.20, No.21-22 (2014), 2817-2829
Suggested Citation
Cameron L. Nemeth, Kajohnkiart Janebodin, Alex E. Yuan, James E. Dennis, Morayma Reyes, Deok Ho Kim Enhanced chondrogenic differentiation of dental pulp stem cells using nanopatterned PEG-GelMA-HA hydrogels. Tissue Engineering - Part A. Vol.20, No.21-22 (2014), 2817-2829. doi:10.1089/ten.tea.2013.0614 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33475
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Title
Enhanced chondrogenic differentiation of dental pulp stem cells using nanopatterned PEG-GelMA-HA hydrogels
Abstract
© 2014, Mary Ann Liebert, Inc. We have examined the effects of surface nanotopography and hyaluronic acid (HA) on in vitro chondrogenesis of dental pulp stem cells (DPSCs). Ultraviolet-assisted capillary force lithography was employed to fabricate well-defined nanostructured scaffolds of composite PEG-GelMA-HA hydrogels that consist of poly(ethylene glycol) dimethacrylate (PEGDMA), methacrylated gelatin (GelMA), and HA. Using this microengineered platform, we first demonstrated that DPSCs formed three-dimensional spheroids, which provide an appropriate environment for in vitro chondrogenic differentiation. We also found that DPSCs cultured on nanopatterned PEG-GelMA-HA scaffolds showed a significant upregulation of the chondrogenic gene markers (Sox9, Alkaline phosphatase, Aggrecan, Procollagen type II, and Procollagen type X), while downregulating the pluripotent stem cell gene, Nanog, and epithelial-mesenchymal genes (Twist, Snail, Slug) compared with tissue culture polystyrene-cultured DPSCs. Immunocytochemistry showed more extensive deposition of collagen type II in DPSCs cultured on the nanopatterned PEG-GelMA-HA scaffolds. These findings suggest that nanotopography and HA provide important cues for promoting chondrogenic differentiation of DPSCs.