Publication: Mosquito densonucleosis virus non-structural protein NS2 is necessary for a productive infection
Issued Date
2008-04-25
Resource Type
ISSN
10960341
00426822
00426822
Other identifier(s)
2-s2.0-41649117506
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Mahidol University
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SCOPUS
Bibliographic Citation
Virology. Vol.374, No.1 (2008), 128-137
Suggested Citation
Eugene Azarkh, Erin Robinson, Supanee Hirunkanokpun, Boris Afanasiev, Pattamaporn Kittayapong, Jonathan Carlson, Joe Corsini Mosquito densonucleosis virus non-structural protein NS2 is necessary for a productive infection. Virology. Vol.374, No.1 (2008), 128-137. doi:10.1016/j.virol.2007.11.035 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19338
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Title
Mosquito densonucleosis virus non-structural protein NS2 is necessary for a productive infection
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Abstract
Mosquito densonucleosis viruses synthesize two non-structural proteins, NS1 and NS2. While NS1 has been studied relatively well, little is known about NS2. Antiserum was raised against a peptide near the N-terminus of NS2, and used to conduct Western blot analysis and immuno-fluorescence assays. Western blots revealed a prominent band near the expected size (41 kDa). Immuno-fluorescence studies of mosquito cells transfected with AeDNV indicate that NS2 has a wider distribution pattern than does NS1, and the distribution pattern appears to be a function of time post-infection. Nuclear localization of NS2 requires intact C-terminus but does not require additional viral proteins. Mutations ranging from complete NS2 knock-out to a single missense amino acid substitution in NS2 can significantly reduce viral replication and production of viable progeny. © 2007 Elsevier Inc. All rights reserved.
