Publication: Blood-feeding and immunogenic Aedes aegypti saliva proteins
Issued Date
2010-05-01
Resource Type
ISSN
16159861
16159853
16159853
Other identifier(s)
2-s2.0-77952375771
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Mahidol University
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SCOPUS
Bibliographic Citation
Proteomics. Vol.10, No.10 (2010), 1906-1916
Suggested Citation
Ladawan Wasinpiyamongkol, Sirilaksana Patramool, Natthanej Luplertlop, Pornapat Surasombatpattana, Souleymane Doucoure, François Mouchet, Martial Séveno, Franck Remoue, Edith Demettre, Jean Paul Brizard, Patrick Jouin, David G. Biron, Frédéric Thomas, Dorothée Missé Blood-feeding and immunogenic Aedes aegypti saliva proteins. Proteomics. Vol.10, No.10 (2010), 1906-1916. doi:10.1002/pmic.200900626 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28727
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Title
Blood-feeding and immunogenic Aedes aegypti saliva proteins
Abstract
Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugar-fed mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was up-regulated. The immunofluorescence assay shows that there is no disruption of the SG cytoskeletal after the blood meal. We have generated for the first time the 2-DE profiles of immunogenic Ae. aegypti SG BF-related proteins. These new data could contribute to the understanding of the physiological processes that appear during the blood meal. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.