Publication: Choline Acetyltransferase in Bovine Pineal Gland
| dc.contributor.author | P. Phansuwan‐Pujito | en_US |
| dc.contributor.author | P. Govitrapong | en_US |
| dc.contributor.author | M. Ebadi | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.contributor.other | University of Nebraska Medical Center, College of Medicine | en_US |
| dc.date.accessioned | 2018-06-14T09:20:19Z | |
| dc.date.available | 2018-06-14T09:20:19Z | |
| dc.date.issued | 1990-01-01 | en_US |
| dc.description.abstract | Recent studies from our laboratories have shown that the bovine pineal gland contains a muscarinic cholinergic receptor with a K d value of 0.423 ± 0.010 nM and a B max value of 69.75 ± 20.91 fmol/mg protein. In order to substantiate further the possible existence of a pineal cholinergic transmission, we have measured the activity of choline acetyltransferase and delineated its kinetic properties in the bovine pineal gland. This enzyme exhibited an activity of 0.0339 ± 0.0042 nmol/mg protein/min. Furthermore, the bovine pineal choline acetyltransferase possessed a K m , value of 124.86 ± 24.06 μM and a V max value of 0.0598 ± 0.0034 nmol/mg protein/min for acetyl CoA, and a K m value of 3.11 ± 0.94 mM and a V max value of 0.0155 ± 0.0016 nmol/mg protein/min for choline. The presence of muscarinic cholinergic receptors along with a specific choline acetyltransferase are supportive evidences that the bovine pineal gland may receive cholinergic innervation. Copyright © 1990, Wiley Blackwell. All rights reserved | en_US |
| dc.identifier.citation | Journal of Pineal Research. Vol.9, No.1 (1990), 29-38 | en_US |
| dc.identifier.doi | 10.1111/j.1600-079X.1990.tb00691.x | en_US |
| dc.identifier.issn | 1600079X | en_US |
| dc.identifier.issn | 07423098 | en_US |
| dc.identifier.other | 2-s2.0-0025033293 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/15923 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0025033293&origin=inward | en_US |
| dc.subject | Biochemistry, Genetics and Molecular Biology | en_US |
| dc.title | Choline Acetyltransferase in Bovine Pineal Gland | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0025033293&origin=inward | en_US |