Publication: A small compound targeting the interaction between nonstructural proteins 2B and 3 inhibits dengue virus replication
Issued Date
2013-10-25
Resource Type
ISSN
10902104
0006291X
0006291X
Other identifier(s)
2-s2.0-84888127965
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.440, No.3 (2013), 393-398
Suggested Citation
Sabar Pambudi, Norihito Kawashita, Supranee Phanthanawiboon, Magot Diata Omokoko, Promsin Masrinoul, Akifumi Yamashita, Kriengsak Limkittikul, Teruo Yasunaga, Tatsuya Takagi, Kazuyoshi Ikuta, Takeshi Kurosu A small compound targeting the interaction between nonstructural proteins 2B and 3 inhibits dengue virus replication. Biochemical and Biophysical Research Communications. Vol.440, No.3 (2013), 393-398. doi:10.1016/j.bbrc.2013.09.078 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31181
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
A small compound targeting the interaction between nonstructural proteins 2B and 3 inhibits dengue virus replication
Other Contributor(s)
Abstract
The non-structural protein NS2B/NS3 serine-protease complex of the dengue virus (DENV) is required for the maturation of the viral polyprotein. Dissociation of the NS2B cofactor from NS3 diminishes the enzymatic activity of the complex. In this study, we identified a small molecule inhibitor that interferes with the interaction between NS2B and NS3 using structure-based screening and a cell-based viral replication assay. A library containing 661,417 small compounds derived from the Molecular Operating Environment lead-like database was docked to the NS2B/NS3 structural model. Thirty-nine compounds with high scores were tested in a secondary screening using a cell-based viral replication assay. SK-12 was found to inhibit replication of all DENV serotypes (EC50=0.74-4.92μM). In silico studies predicted that SK-12 pre-occupies the NS2B-binding site of NS3. Steady-state kinetics using a fluorogenic short peptide substrate demonstrated that SK-12 is a noncompetitive inhibitor against the NS2B/NS3 protease. Inhibition to Japanese encephalitis virus by SK-12 was relatively weak (EC50=29.81μM), and this lower sensitivity was due to difference in amino acid at position 27 of NS3. SK-12 is the promising small-molecule inhibitor that targets the interaction between NS2B and NS3. © 2013 Elsevier Inc.