Publication: Transplantation of adipose tissue-derived stem cell sheet to reduce leakage after partial colectomy in a rat model
1
Issued Date
2018-08-11
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ISSN
1940087X
DOI
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2-s2.0-85054543259
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Visualized Experiments. Vol.2018, No.138 (2018)
Suggested Citation
Panithi Sukho, Geesien S.A. Boersema, Nicole Kops, Johan F. Lange, Jolle Kirpensteijn, Jan Willem Hesselink, Yvonne M. Bastiaansen-Jenniskens, Femke Verseijden Transplantation of adipose tissue-derived stem cell sheet to reduce leakage after partial colectomy in a rat model. Journal of Visualized Experiments. Vol.2018, No.138 (2018). doi:10.3791/57213 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/45083
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Title
Transplantation of adipose tissue-derived stem cell sheet to reduce leakage after partial colectomy in a rat model
Abstract
© 2018 Journal of Visualized Experiments. Anastomotic leakage is a disastrous complication after colorectal surgery. Although current methods for leakage prevention have different levels of clinical efficacy, they are until now imperfect solutions. Stem cell therapy using ASC sheets could provide a solution to this problem. ASCs are considered as promising candidates for promoting tissue healing because of their trophic and immunomodulatory properties. Here, we provide methods to produce high-density ASC sheets, that are transplanted onto a colorectal anastomosis in a rat model to reduce the leakage. ASCs formed cell sheets in thermo-responsive culture dishes that could be easily detached. On the day of the transplantation, a partial colectomy with a 5-suture colorectal anastomosis was performed. Animals were immediately transplanted with 1 ASC sheet per rat. ASC sheets adhered spontaneously to the anastomosis without any glue, suture, or any biomaterial. Animal groups were sacrificed 3 and 7 days postoperatively. Compared to transplanted animals, the incidence of anastomotic abscesses and leakage was higher in control animals. In our model, the transplantation of ASC sheets after colorectal anastomosis was successful and associated with a lower leakage rate.