Publication: Phenotypic characterization of circulating CD4/CD8 t-lymphocytes in β-thalassemia patients
2
Issued Date
2014-01-01
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ISSN
22288694
0125877X
0125877X
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2-s2.0-84911900587
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.32, No.3 (2014), 261-269
Suggested Citation
Egarit Noulsri, Surada Lerdwana, Suthat Fucharoen, Kovit Pattanapanyasat Phenotypic characterization of circulating CD4/CD8 t-lymphocytes in β-thalassemia patients. Asian Pacific Journal of Allergy and Immunology. Vol.32, No.3 (2014), 261-269. doi:10.12932/AP0426.32.3.2014 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/34044
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Title
Phenotypic characterization of circulating CD4/CD8 t-lymphocytes in β-thalassemia patients
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Abstract
© 2014, Allergy and Immunology Society of Thailand. All rights reserved. Background: Infection is one of the most common causes of death in β-thalassemia patients. This may be due in part to an underlying immunological abnormality. During the past decade, a subset of CD3+ T cells that express both CD4+CD8+ (DP) T-cells were discovered and have been described in several pathological conditions. However, phenotypic characterization of this unique T-lymphocyte subset in patients with β -thalassemia has not yet been investigated.Methods: Flow cytometry was used to determine the frequency of such CD4+CD8+(DP) cells in concert with frequencies of CD4+, CD8+, NKT cells and γδ-TCR T-lymphocytes in the peripheral blood of β-thalassemia/HbE patients. The frequencies of these lymphocyte subsets were compared with those in blood samples from healthy volunteers.Results: The results showed that the frequency of lymphocytes was significantly increased in splenectomized β-thalassemia/HbE patients but the frequencies of CD3+, CD4+ and CD8+ T-lymphocytes were not significantly different among the studied groups. However, analysis of unconventional T-lymphocytes revealed a significant increase in the frequency of CD4-CD8- in splenectomized β-thalassemia/HbE patients. The frequencies of CD4-CD8dim and CD4+CD8+ in β-thalassemia/HbE patients were similar to the controls. Further classification of the CD4+CD8+ cells revealed that β-thalassemia/HbE patient expressed significantlyhigh levels of CD4brightCD8dim, with a marked increase found in non-splenectomized patients. Furthermore, significant increases in the frequency of γδ -TCR and NKT cells were also demonstrated in these splenectomized β-thalassemia/HbE patients.Conclusion: Our findings show the alteration of unconventional T-lymphocyte subsets in β-thalassemia/HbE patients, which may be responsible or may reflect the impaired immune response in β-thalssemia disease.