Publication: Molecular detection of Schistosoma japonicum in infected snails and mouse faeces using a real-time PCR assay with FRET hybridisation probes
Issued Date
2011-11-01
Resource Type
ISSN
16788060
00740276
00740276
Other identifier(s)
2-s2.0-82255169684
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Memorias do Instituto Oswaldo Cruz. Vol.106, No.7 (2011), 831-836
Suggested Citation
Tongjit Thanchomnang, Pewpan Intapan, Pusadee Sri-Aroon, Viraphong Lulitanond, Penchome Janwan, Oranuch Sanpool, Wanchai Maleewong Molecular detection of Schistosoma japonicum in infected snails and mouse faeces using a real-time PCR assay with FRET hybridisation probes. Memorias do Instituto Oswaldo Cruz. Vol.106, No.7 (2011), 831-836. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/12244
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Molecular detection of Schistosoma japonicum in infected snails and mouse faeces using a real-time PCR assay with FRET hybridisation probes
Other Contributor(s)
Abstract
A real-time polymerase chain reaction (PCR) assay with fluorescence resonance energy transfer (FRET) hybridisation probes combined with melting curve analysis was developed to detect Schistosoma japonicum in experimentally infected snails and in faecal samples of infected mice. This procedure is based on melting curve analysis of a hybrid between an amplicon from the S. japonicum internal transcribed spacer region 2 sequence, which is a 192-bp S. japonicum-specific sequence, and fluorophore-labelled specific probes. Real-time FRET PCR could detect as little as a single cercaria artificially introduced into a pool of 10 non-infected snails and a single egg inoculated in 100 mg of non-infected mouse faeces. All S. japonicum-infected snails and all faecal samples from infected mice were positive. Non-infected snails, non-infected mouse faeces and genomic DNA from other parasites were negative. This assay is rapid and has potential for epidemiological S. japonicum surveys in snails, intermediate hosts and faecal samples of final hosts.