Publication: siRNA-mediated silencing of the 37/67-kDa high affinity laminin receptor in Hep3B cells induces apoptosis
Issued Date
2008-01-01
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ISSN
16891392
14258153
14258153
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2-s2.0-56349171792
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Mahidol University
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SCOPUS
Bibliographic Citation
Cellular and Molecular Biology Letters. Vol.13, No.3 (2008), 452-454
Suggested Citation
Tharinee Susantad, Duncan R. Smith siRNA-mediated silencing of the 37/67-kDa high affinity laminin receptor in Hep3B cells induces apoptosis. Cellular and Molecular Biology Letters. Vol.13, No.3 (2008), 452-454. doi:10.2478/s11658-008-0017-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18990
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Title
siRNA-mediated silencing of the 37/67-kDa high affinity laminin receptor in Hep3B cells induces apoptosis
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Abstract
The laminin-binding protein, variously called the 37/67-kDa high affinity laminin receptor or p40, mediates the attachment of normal cells to the laminin network, and also has a role as a ribosomal protein. Over-expression of this protein has been strongly correlated with the metastatic phenotype. However, few studies have investigated the cellular consequence of the ablation of this gene's expression. To address this issue, the expression of the 37/67-kDa high affinity laminin receptor was knocked out with several siRNA constructs via RNA interference in transformed liver (Hep3B) cells. In each case where the message was specifically ablated, apoptosis was induced, as determined by annexin V/propidium iodide staining, and by double staining with annexin V and an antibody directed against the 37/67-kDa high affinity laminin receptor. These results suggest that this protein plays a critical role in maintaining cell viability. © 2008 by the University of Wrocław.