Publication: Chitosan-functionalized poly(methyl methacrylate) particles by spinning disk processing for lipase immobilization
Issued Date
2012-07-01
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ISSN
01448617
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2-s2.0-84861589672
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Mahidol University
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SCOPUS
Bibliographic Citation
Carbohydrate Polymers. Vol.89, No.3 (2012), 842-848
Suggested Citation
Somkieath Jenjob, Panya Sunintaboon, Pranee Inprakhon, Natthinee Anantachoke, Vichai Reutrakul Chitosan-functionalized poly(methyl methacrylate) particles by spinning disk processing for lipase immobilization. Carbohydrate Polymers. Vol.89, No.3 (2012), 842-848. doi:10.1016/j.carbpol.2012.04.019 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13959
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Title
Chitosan-functionalized poly(methyl methacrylate) particles by spinning disk processing for lipase immobilization
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Abstract
Chitosan-functionalized poly(methyl methacrylate) (PMMA-CH) particles were prepared by complexation between the negatively charged PMMA particles and the positively charged chitosan via a spinning disk processing. Processing parameters; feed rate and spinning speed, were optimized, which were traced by size distribution profiles of the formed PMMA-CH particles. Their sizes and net surface charges were found to be affected by MWs of chitosan (45, 100, and 230 kDa) used. Microscopic evidences were used to confirm their core-shell morphology. Chemical characteristics and thermal stability of such particles were determined by FTIR and TGA, respectively. Then, their ability to immobilize lipase (EC 3.1.1.3) was conducted and followed through zeta potential measurement. The percentage of lipase adsorption capacity increased with increasing lipase content, but the value decreased when the size of PMMA-CH particles in creased. Also, the activity of lipase attached on PMMA-CH particles' surface was preserved and increased with lipase loading. © 2012 Elsevier Ltd. All rights reserved.