Publication: Staphylococcus aureus clinical isolates: Antibiotic susceptibility, molecular characteristics, and ability to form biofilm
Issued Date
2013-09-23
Resource Type
ISSN
23146141
23146133
23146133
Other identifier(s)
2-s2.0-84884240870
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
BioMed Research International. Vol.2013, (2013)
Suggested Citation
N. Indrawattana, O. Sungkhachat, N. Sookrung, M. Chongsa-Nguan, A. Tungtrongchitr, S. P. Voravuthikunchai, T. Kong-Ngoen, H. Kurazono, W. Chaicumpa Staphylococcus aureus clinical isolates: Antibiotic susceptibility, molecular characteristics, and ability to form biofilm. BioMed Research International. Vol.2013, (2013). doi:10.1155/2013/314654 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31212
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Staphylococcus aureus clinical isolates: Antibiotic susceptibility, molecular characteristics, and ability to form biofilm
Abstract
Periodic monitoring of Staphylococcus aureus characteristics in a locality is imperative as their drug-resistant variants cause treatment problem. In this study, antibiograms, prevalence of toxin genes (sea-see, seg-ser, seu, tsst-1, eta, etb, and etd), PFGE types, accessory gene regulator (agr) groups, and ability to form biofilm of 92 S. aureus Thailand clinical isolates were investigated. They were classified into 10 drug groups: groups 1-7 (56 isolates) were methicillin resistant (MRSA) and 8-10 (36 isolates) were methicillin sensitive (MSSA). One isolate did not have any toxin gene, 4 isolates carried one toxin gene (seq), and 87 isolates had two or more toxin genes. No isolate had see, etb, or tsst-1; six isolates had eta or etd. Combined seg-sei-sem-sen-seo of the highly prevalent egc locus was 26.1%. The seb, sec, sel, seu, and eta associated significantly with MSSA; sek was more in MRSA. The sek-seq association was 52.17% while combined sed-sej was not found. Twenty-three PFGE types were revealed, no association of toxin genes with PFGE types. All four agr groups were present; agr group 1 was predominant (58.70%) but agr group 2 strains carried more toxin genes and were more frequent toxin producers. Biofilm formation was found in 72.83% of the isolates but there was no association with antibiograms. This study provides insight information on molecular and phenotypic markers of Thailand S. aureus clinical isolates which should be useful for future active surveillance that aimed to control a spread of existing antimicrobial resistant bacteria and early recognition of a newly emerged variant. © 2013 N. Indrawattana et al.