Publication: Tools for detection of Mycoplasma amphoriforme: A primary respiratory pathogen?
Issued Date
2014-01-01
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ISSN
1098660X
00951137
00951137
Other identifier(s)
2-s2.0-84897145728
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Clinical Microbiology. Vol.52, No.4 (2014), 1177-1181
Suggested Citation
Clare L. Ling, Katarina Oravcova, Thomas F. Beattie, Dean D. Creer, Paul Dilworth, Naomi L. Fulton, Alison Hardie, Michelle Munro, Marcus Pond, Kate Templeton, David Webster, Sarita Workman, Timothy D. McHugh, Stephen H. Gillespie Tools for detection of Mycoplasma amphoriforme: A primary respiratory pathogen?. Journal of Clinical Microbiology. Vol.52, No.4 (2014), 1177-1181. doi:10.1128/JCM.03049-13 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/34852
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Title
Tools for detection of Mycoplasma amphoriforme: A primary respiratory pathogen?
Abstract
Mycoplasma amphoriforme is a recently described organism isolated from the respiratory tracts of patients with immunodeficiency and evidence of chronic infection. Novel assays for the molecular detection of the organism by real-time quantitative PCRs (qPCRs) targeting the uracil DNA glycosylase gene ( udg) or the 23S rRNA gene are described here. The analytical sensitivities are similar to the existing conventional M. amphoriforme 16S rRNA gene PCR, with the advantage of being species specific, rapid, and quantitative. By using these techniques, we demonstrate the presence of this organism in 17 (19.3%) primary anti-body-deficient (PAD) patients, 4 (5%) adults with lower respiratory tract infection, 1 (2.6%) sputum sample from a patient attending a chest clinic, and 23 (0.21%) samples submitted for viral diagnosis of respiratory infection, but not in normal adult control subjects. These data show the presence of this microorganism in respiratory patients and suggest that M. amphoriforme may infect both immunocompetent and immunocompromised people. Further studies to characterize this organism are required, and this report provides the tools that may be used by other research groups to investigate its pathogenic potential. Copyright © 2014 American Society for Microbiology. All Rights Reserved.