Publication: Proteome analyses of cellular proteins in methicillin-resistant staphylococcus aureus treated with rhodomyrtone, a novel antibiotic Candidate
Issued Date
2011
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eng
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Mahidol University
Bibliographic Citation
Plos One. Vol.6, No.2 (2011), e16628
Suggested Citation
Wipawadee Sianglum, Potjanee Srimanote, Wijit Wonglumsom, Kanokwan Kittiniyom, Supayang P.Voravuthikunchai Proteome analyses of cellular proteins in methicillin-resistant staphylococcus aureus treated with rhodomyrtone, a novel antibiotic Candidate. Plos One. Vol.6, No.2 (2011), e16628. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2071
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Title
Proteome analyses of cellular proteins in methicillin-resistant staphylococcus aureus treated with rhodomyrtone, a novel antibiotic Candidate
Abstract
The ethanolic extract from Rhodomyrtus tomentosa leaf exhibited good antibacterial activities against both methicillinresistant
Staphylococcus aureus (MRSA) and S. aureus ATCC 29213. Its minimal inhibitory concentration (MIC) values ranged
from 31.25–62.5 mg/ml, and the minimal bactericidal concentration (MBC) was 250 mg/ml. Rhodomyrtone, an
acylphloroglucinol derivative, was 62.5–125 times more potent at inhibiting the bacteria than the ethanolic extract, the
MIC and MBC values were 0.5 mg/ml and 2 mg/ml, respectively. To provide insights into antibacterial mechanisms involved,
the effects of rhodomyrtone on cellular protein expression of MRSA have been investigated using proteomic approaches.
Proteome analyses revealed that rhodomyrtone at subinhibitory concentration (0.174 mg/ml) affected the expression of
several major functional classes of whole cell proteins in MRSA. The identified proteins involve in cell wall biosynthesis and cell division, protein degradation, stress response and oxidative stress, cell surface antigen and virulence factor, and various metabolic pathways such as amino acid, carbohydrate, energy, lipid, and nucleotide metabolism. Transmission electron
micrographs confirmed the effects of rhodomyrtone on morphological and ultrastructural alterations in the treated bacterial cells. Biological processes in cell wall biosynthesis and cell division were interrupted. Prominent changes including
alterations in cell wall, abnormal septum formation, cellular disintegration, and cell lysis were observed. Unusual size and
shape of staphylococcal cells were obviously noted in the treated MRSA. These pioneer findings on proteomic profiling and
phenotypic features of rhodomyrtone-treated MRSA may resolve its antimicrobial mechanisms which could lead to the
development of a new effective regimen for the treatment of MRSA infections.