Publication: Shewanella khirikhana sp. nov. – a shrimp pathogen isolated from a cultivation pond exhibiting early mortality syndrome
Issued Date
2020-01-01
Resource Type
ISSN
17517915
17517907
17517907
Other identifier(s)
2-s2.0-85078739526
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microbial Biotechnology. (2020)
Suggested Citation
Anuphap Prachumwat, Piyanuch Wechprasit, Jiraporn Srisala, Ruttanaporn Kriangsaksri, Timothy W. Flegel, Siripong Thitamadee, Kallaya Sritunyalucksana Shewanella khirikhana sp. nov. – a shrimp pathogen isolated from a cultivation pond exhibiting early mortality syndrome. Microbial Biotechnology. (2020). doi:10.1111/1751-7915.13538 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/53608
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Shewanella khirikhana sp. nov. – a shrimp pathogen isolated from a cultivation pond exhibiting early mortality syndrome
Other Contributor(s)
Abstract
© 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. Early mortality syndrome (EMS) in cultivated shrimp is of complex aetiology. One of the causes is acute hepatopancreatic necrosis disease (AHPND) caused by unique Vibrio isolates that carry two Pirvp toxin genes, but other causes of EMS remain mostly unexplained. Here, we describe the discovery of a Shewanella isolate TH2012T from an EMS/AHPND outbreak pond and demonstrate its virulence for shrimp (the mean lethal concentration of 105 colony-forming units per millilitre by immersion challenge) accompanied by distinctive histopathology, particularly of the ventral nerve cord and lymphoid organ but also including the digestive tract. On the basis of its complete genome sequence, multilocus phylogenetic trees, digital DNA–DNA hybridization analysis and differential phenotypic characteristics, we propose that Shewanella isolate TH2012T represents a novel species, separated sufficiently from the type strains S. litorisediminis and S. amazonensis to justify naming it Shewanella khirikhana sp. nov. Analysis of the TH2012T genome revealed no homologues of the Pirvp toxin genes but revealed a number of other potential virulence factors. It constitutes the first Shewanella isolate reported to be pathogenic to shrimp.