Publication: Trafficking defect of mutant kidney anion exchanger 1 (kAE1) proteins associated with distal renal tubular acidosis and Southeast Asian ovalocytosis
Issued Date
2006-11-24
Resource Type
ISSN
10902104
0006291X
0006291X
Other identifier(s)
2-s2.0-33750002472
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.350, No.3 (2006), 723-730
Suggested Citation
Nunghathai Sawasdee, Wandee Udomchaiprasertkul, Sansanee Noisakran, Nanyawan Rungroj, Varaporn Akkarapatumwong, Pa thai Yenchitsomanus Trafficking defect of mutant kidney anion exchanger 1 (kAE1) proteins associated with distal renal tubular acidosis and Southeast Asian ovalocytosis. Biochemical and Biophysical Research Communications. Vol.350, No.3 (2006), 723-730. doi:10.1016/j.bbrc.2006.09.113 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22947
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Title
Trafficking defect of mutant kidney anion exchanger 1 (kAE1) proteins associated with distal renal tubular acidosis and Southeast Asian ovalocytosis
Abstract
Compound heterozygous anion exchanger 1 (AE1) SAO/G701D mutations result in distal renal tubular acidosis with Southeast Asian ovalocytosis. Interaction, trafficking and localization of wild-type and mutant (SAO and G701D) kAE1 proteins fused with hemagglutinin, six-histidine, Myc, or green fluorescence protein (GFP) were examined in human embryonic kidney (HEK) 293 cells. When individually expressed, wild-type kAE1 was localized at cell surface while mutant kAE1 SAO and G701D were intracellularly retained. When co-expressed, wild-type kAE1 could form heterodimer with kAE1 SAO or kAE1 G701D and could rescue mutant kAE1 proteins to express on the cell surface. Co-expression of kAE1 SAO and kAE1 G701D also resulted in heterodimer formation but intracellular retention without cell surface expression, suggesting their trafficking defect and failure to rescue each other to the plasma membrane, most likely the molecular mechanism of the disease in the compound heterozygous condition. © 2006 Elsevier Inc. All rights reserved.