Publication: Analysis of N-glycan profile of Arabidopsis alg3 cell cultur
Issued Date
2021-01-01
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ISSN
13476114
13424580
13424580
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2-s2.0-85124017471
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Mahidol University
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SCOPUS
Bibliographic Citation
Plant Biotechnology. Vol.38, No.4 (2021), 463-467
Suggested Citation
Ratna Sariyatun, Hiroyuki Kajiura, Juthamard Limkul, Ryo Misaki, Kazuhito Fujiyama Analysis of N-glycan profile of Arabidopsis alg3 cell cultur. Plant Biotechnology. Vol.38, No.4 (2021), 463-467. doi:10.5511/PLANTBIOTECHNOLOGY.21.1025A Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/75751
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Title
Analysis of N-glycan profile of Arabidopsis alg3 cell cultur
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Abstract
N-Glycosylation is essential for protein stability, activity and characteristics, and is often needed to deliver pharmaceutical glycoproteins to target cells. A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases. However, M3 is generally produced at a very low level in plants. In this study, a cell culture was established from an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity. Arabidopsis alg3 cell culture produced glycoproteins with predominantly M3 and GlcNAc-terminal structures, while the amount of plant-specific N-glycans was very low. Pharmaceutical glycoproteins with these characteristics would be valuable for cellular delivery through the MR, and safe for human therapy.