Publication: A simple monochromatic flow cytometric assay for assessment of intraerythrocytic development of Plasmodium falciparum
Issued Date
2020-02-18
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14752875
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2-s2.0-85079745191
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Mahidol University
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SCOPUS
Bibliographic Citation
Malaria Journal. Vol.19, No.1 (2020)
Suggested Citation
Kasem Kulkeaw, Nutpakal Ketprasit, Anchalee Tungtrongchitr, Duangdao Palasuwan A simple monochromatic flow cytometric assay for assessment of intraerythrocytic development of Plasmodium falciparum. Malaria Journal. Vol.19, No.1 (2020). doi:10.1186/s12936-020-03156-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/53695
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Title
A simple monochromatic flow cytometric assay for assessment of intraerythrocytic development of Plasmodium falciparum
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Abstract
© 2020 The Author(s). Background: Gold standard microscopic examination of Plasmodium falciparum intraerythrocytic stage remains an important process for staging and enumerating parasitized erythrocytes in culture; however, microscopy is laborious and its accuracy is dependent upon the skill of the examiner. Methods: In this study, ViSafe Green (VSG), which is a nucleic acid-binding fluorescent dye, was used for assessing in vitro development of P. falciparum using flow cytometry. Results: Fluorescence intensity of VSG was found to depend on the developmental stage of parasites. Specifically, multiple-nuclei-containing schizonts were observed in the VSGhigh population, and growing trophozoites and ring-shaped forms were observed in the VSGintermediate and VSGlow populations. The efficacy of VSG-based assay was found to be comparable to the microscopic examination method, and it demonstrated an ability to detect as low as 0.001% of the parasitaemia estimated by Giemsa staining. Moreover, when applying VSG for anti-malarial drug test, it was able to observe the growth inhibitory effect of dihydroartemisinin, the front-line drug for malaria therapy. Conclusions: Taken together, the results of this study suggest the VSG-based flow cytometric assay to be a simple and reliable assay for assessing P. falciparum malaria development in vitro.