Publication: Platelet-rich plasma stimulated proliferation, migration, and attachment of cultured periodontal ligament cells
Issued Date
2018-10-01
Resource Type
ISSN
13057464
13057456
13057456
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2-s2.0-85054505169
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Mahidol University
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SCOPUS
Bibliographic Citation
European Journal of Dentistry. Vol.12, No.4 (2018), 469-474
Suggested Citation
Kanyawat Rattanasuwan, Supanee Rassameemasmaung, Sirichai Kiattavorncharoen, Anongporn Sirikulsathean, Jarinee Thorsuwan, Wilasinee Wongsankakorn Platelet-rich plasma stimulated proliferation, migration, and attachment of cultured periodontal ligament cells. European Journal of Dentistry. Vol.12, No.4 (2018), 469-474. doi:10.4103/ejd.ejd_255_17 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45698
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Title
Platelet-rich plasma stimulated proliferation, migration, and attachment of cultured periodontal ligament cells
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Abstract
© 2018 European Journal of Dentistry | Published by Wolters Kluwer - Medknow. Objective: The aim of this study is to evaluate the effects of platelet-rich plasma (PRP) on the proliferation, migration, and attachment of cultured periodontal ligament (PDL) cells. Materials and Methods: 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to assess number of PDL cells cultured in medium with or without PRP. Cell migration toward medium with or without PRP was assessed using the Boyden chamber. Cell attachment was assessed by counting cells on PRP or non-PRP coated dentin specimens. Group differences were analyzed using two-way ANOVA at 0.05 significance level. Results: In the MTT and cell migration assay, the number of cells in 5% and 10% PRP-treated groups were significantly higher than that in the non-PRP-treated group (P < 0.05). In the attachment assay, the number of cells on the dentin specimens in 10% PRP-treated group was significantly higher than that in the non-PRP treated group (P < 0.05). Conclusion: PRP could stimulate proliferation, migration, and attachment of PDL cells.